Human Anti-Cetuximab Antibody, clone AbD19834
货号：HCA220
规格：0.1 mg
价格：4522
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Cetuximab
物种：其它
宿主：人
抗体亚型：其它
克隆号：AbD19834
荧光染料：其它
| 产品介绍 | | |
| Human Anti-Cetuximab Antibody, clone AbD19834, is an anti-idiotypic antibody that binds specifically to cetuximab and inhibits the binding of the drug to its target, epidermal growth factor receptor (EGFR). It can be used in bioanalytical assays to measure the levels of cetuximab and biosimilar products in preclinical research and clinical trials.Clone AbD19834 is recommended as the capture antibody in a pharmacokinetic (PK) bridging ELISA. When paired with another inhibitory antibody, HRP conjugated Anti-Cetuximab Antibody clone AbD19830_hIgG1 (HCA221P), as detection antibody, free drug can be measured. When paired with the non-inhibitory Anti-Cetuximab Antibody, clone AbD19376_hIgG1 (HCA228P), free and partially bound drug can be measured, which is equivalent to total drug when trough serum levels are above 4 μg/ml.Cetuximab (brand name Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor. |
| 产品详情 | | |
| Product Form | A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed inE. coli.The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid. |
| Preparation | Metal chelate affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Cetuximab. |
| Affinity | The monovalent intrinsic affinity of this antibody was measured as KD= 0.5 nM by real time, label free molecular interaction analysis on immobilized cetuximab. |
| Approx. Protein ConcePurityntrations | Total protein concentration 0.5 mg/ml |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.Erbitux® is a registered trademark of ImClone LLC, a wholly-owned subsidiary of Eli Lilly and Company.His-tag is a registered trademark of EMD Biosciences. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E | 0.1mg |
技术参数 产品应用 elisa
产品类型 单克隆抗体
推荐用于 elisa
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