FLISP™ FAM-Leu-CMK Serine Protease Assay Kit
货号：APO007
规格：25 Tests
价格：3596
产品类型：检测试剂盒
品牌：Bio-Rad
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| 产品介绍 | | |
| Fluorescent Labeled Inhibitors of Serine Protease (FLISP) kits allow the analysis of intracellular chymotrypsin-like serine protease activity in live cells. Serine protease activation plays major roles in several different cellular functions including apoptosis, tumor malignancy and as diagnostic and prognostic indicators of breast, head and neck carcinomas. Serine proteases are also thought to play a role in infections and transplant rejection. |
| 产品详情 | | |
| Test Principle | Active chymotrypsin-like enzymes within the cell bind covalently to the FLISP FAM-Leu-CMK inhibitor probe, retaining the green fluorescent signal within the cell. The higher the concentration of active chymotrypsin-like enzymes within the cell, the higher the level of fluorescence observed. FLISP kits are suitable for use with suspension or adherent cells and can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader.FLISP inhibitors are cell-permeable and non-cytotoxic fluorochrome-labeled analogs of the first serine protease inhibitor, tosyl-phenlalanylchloromethyl ketone (TPCK), labeled with carboxyfluorescein (FAM) which fluoresces green. They are available with either a chloromethyl ketone (CMK) or diphenyl 1-(N-peptidylamino) alkanephosphonate (DAP) reactive group containing compound. Specificity for chymotrypsin-like enzymes is determined by the amino acid targeting peptide, the FAM-Leu-CMK probe inhibits chymotrypsin C. |
| Reagents in the Kit | FAM-Phe-CMK FLISP Reagent - lyophilized |
| 10X Wash Buffer | 15 mL |
| Fixative | 6 mL |
| Propidium Iodide | 1 mL |
| Hoechst Stain | 1 mL |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FAM | 494 | 520 |
| Regulatory | For research purposes only |
| Acknowledgements | FLISP is a trademark of Immunochemistry Technologies, LLC. |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label.Store the FLISP reagent at -20℃. Once reconstituted in DMSO, the FLISP stock should be stored at -20℃for 6 months, protected from light. Avoid repeated freezing and thawing. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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产品类型 检测试剂盒
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