| 产品介绍 | | |
| Rabbit anti Human Mcl-1 antibodyrecognizes an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours.The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival.Rabbit anti Human Mcl-1 antibody (AHP998) recognizes both the MCL-1L (~38 kDa) and MCL-1S (~30 kDa) isoforms of MCL-1. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Antiserum Preparation | Antisera to human MCL-1 (IN) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography. |
| Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
| Immunogen | A 16 amino acid peptide located near the centre of human MCL-1. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 0.5 | 1.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.ozopas, K.M.et al.(1993) MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2.Proc Natl Acad Sci U S A. 90 (8): 3516-20.2.Bae, J.et al.(2000) MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.J Biol Chem. 275 (33): 25255-61.3.Rinkenberger, J.L.et al.(2000) Mcl-1 deficiency results in peri-implantation embryonic lethality.Genes Dev. 14 (1): 23-7.4.Michels, J.et al.(2005) Mcl-1.Int J Biochem Cell Biol. 37 (2): 267-71. |
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