| 产品介绍 | | |
| Rabbit anti GAPDH (C-Terminal) antibodyrecognizes an epitope within the C-Terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate.GAPDH has a role as a multifunctional protein and is involved in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimers disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdons disease) and the androgen receptor (spinobulbar muscular atrophy), indicates roles for GAPDH in the pathogenesis of neurodegenerative diseases (De Santoet al.2010). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Antiserum Preparation | Antisera to human GAPDH (CT) were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG prepared by affinity chromatography. |
| Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
| Immunogen | A 16 amino acid peptide located near human GAPDH carboxy-terminus. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 0.5 | 1.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.De Santo, C.et al.(2010) Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A.Nat Immunol. 11 (11): 1039-46.2.Weeke-Klimp, A.et al.(2010) Epicardium-derived cells enhance proliferation, cellular maturation and alignment of cardiomyocytes.J Mol Cell Cardiol. 49: 606-16.3.Deacon, K. and Knox, A.J. (2010) Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop.J Biol Chem. 285: 25913-27.4.Li, J.et al.(2020) Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLDEvidence-Based Complementary and Alternative Medicine. 2020: 1-15. |
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