Rabbit anti Human Caspase-5 antibody
货号：AHP965
规格：0.1 mg
价格：4692
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Caspase-5
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human Caspase-5 antibodyrecognizes an epitope located within the internal region of human Caspase-5, a ~47 kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.Thein vivoexpression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma.In vitrocleavage of caspase-5 by the serine protease granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis (Wanget al.1996) |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.02%Sodium Azide |
| Immunogen | A 14 amino acid peptide from the centre of human Caspase-5. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1.0ug/ml | 2.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Wang, S.et al.(1996) Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE.J Biol Chem. 271 (34): 20580-7.2.Lin, X.Y.et al.(2000) Expression analysis of the human caspase-1 subfamily reveals specific regulation of the CASP5 gene by lipopolysaccharide and interferon-gamma.J Biol Chem. 275 (51): 39920-6.3.Munday, N.A.et al.(1995) Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.J Biol Chem. 270 (26): 15870-6.4.Cohen, G.M. (1997) Caspases: the executioners of apoptosis.Biochem J. 326 ( Pt 1): 1-16. |
技术参数 产品应用 wb
产品类型 多克隆抗体
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