| 产品介绍 | | |
| Rabbit anti Human EGFrecognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumour cells (Senoet al.1996). Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Immunogen | Recombinant human EGF (PHP030A). |
| Reconstitution | Reconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add azide.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
| Antiserum Preparation | Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.Purified IgG prepared by affinity chromatography. |
| Preservative Stabilisers | None present. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.J Biol Chem. 265 (14): 7709-12. |
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