产品介绍 |
| Rabbit anti 14-3-3 sigma antibodyrecognizes 14-3-3 sigma, also known as SFN, YWHAS or stratifin. 14-3-3 Sigma is a member of the 14-3-3 family of proteins. 14-3-3 proteins regulate multiple cell signaling pathways through their interaction with discrete protein sequences. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Mouse √ |
| Rat √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to 14-3-3 sigma was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
Immunogen | Recombinant human 14-3-3 sigma |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | 1/50 | 1/200 |
Immunohistology - Paraffin | √ | 1/50 | 1/200 |
Western Blotting | √ | 1/500 | 1/2000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti 14-3-3 sigma antibody detects a band of approximately 28 kDa in cell lysates under reducing conditions
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