产品介绍 |
| Rabbit anti caspase-9 p10 antibodyrecognizes caspase-9, a member of the cysteine-aspartic acid protease family. The active form of caspase-9 is generated by cleavage of the pro-caspase-9 protein into fragments, known as the large and small fragments. The trigger for caspase-9 cleavage is the formation of the apoptosome (comprising of cytochrome c, APAF-1, dATP and pro-caspase-9), upon stimulation of the intrinsic apoptosis pathway.The rabbit anti caspase-9 antibody was raised against an internal peptide that is present in one of the small fragments (p10) generated in the pro-caspase-9 cleavage process at Asp330 (Zouet al.2002). The antibody will therefore detect pro-caspase-9 (as the motif is present) and the p10 caspase-9 fragment. The antibody can therefore be used to distinguish the p10 caspase-9 fragment from other pro-caspase-9 cleavage products. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target SpeciesCross Reactivity |
| Mouse √ |
| Rat √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography |
AntiserumPreparation | Antiserum to caspase-9 p10 was raised by repeated immunisation of rabbits with highly purified antigen |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3)0.2% Gelatin |
Immunogen | Peptide mapping to an internal region of human caspase-9 p10 subunit |
Approx. Protein Concentrations | IgG concentration 0.2 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1/200 | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti caspase-9 p10 recognizes a band of approximately 47 kDa when run under reducing conditions without induction of apoptosis
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