产品介绍 |
| Rabbit anti BrdU antibodyrecognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.BrdU is also commonly usedin vivofor monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqziet al. 2004) or in drinking water (Moseret al. 2004). |
产品详情 |
Target Species | Chemical |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | Bromodeoxyuridine conjugated to KLH |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry1 | √ | 1/50 | 1/100 |
Immunocytochemistry | √ | | 1/500 |
Immunofluorescence2 | √ | 1/100 | 1/500 |
Immunohistology - Paraffin | √ | 1/100 | 1/500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Flow Cytometry protocols can be found at:BrdU Staining of Cells with Rabbit Anti-BrdU Antibody by Flow Cytometry.2BrdU labeling and immunostaining protocol can be found at:www.bio-rad-antibodies.com/rabbit-brdu-labeling-and-immunostaining-protocolWhere this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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