产品介绍 |
| Rabbit anti GATA3 antibodyrecognizes GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (uniprot P15976).GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Hoet al.1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chouet al. 2010).Aberrant GATA3 levels have been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Kringset al.2014). |
产品详情 |
Target Species | Human |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to GATA3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3)1 mg/ml Bovine Serum Albumin30% Glycerol |
Immunogen | Purified partial recombinant GATA3 protein expressed inE. coli |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Immunocytochemistry | √ | 1/50 | 1/500 |
Immunofluorescence | √ | 1/50 | 1/500 |
Immunohistology - Paraffin1 | √ | 1/50 | 1/500 |
Western Blotting | √ | | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH6.0 for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti GATA3 antibody recognizes a band of approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract
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