| 产品介绍 | | |
| Mouse anti Pig SLA Class II DQ antibody, clone K274.3G8recognizes SLA DQ molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR. |
| 产品详情 | | |
Target Species | Pig |
| Product Form | Purified IgG - liquid |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Bovine | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Porcine peripheral blood lymphocytes |
| Approx. Protein Concentrations | IgG concentration 1.05 mg/ml |
Fusion Partners | Spleen cells from immunized mice were fused with cells of the P3-X63-Ag.653 myeloma cell line |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/10 | 1/100 |
| Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells in 100ul . |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests/1ml |
| 数据 |
| Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q (MCA1335GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F). |
| 文献 |
| 1. Weesendorp Eet al.(2013) Phenotypic modulation and cytokine profiles of antigen presenting cells by European subtype 1 and 3 porcine reproductive and respiratory syndrome virus strainsin vitroandin vivo.Vet Microbiol. 167 (3-4): 638-50.2. Makala, L.H.et al.(2001) Ontogeny of pig discrete Peyer's patches: expression of surface antigens.J Vet Med Sci. 63 (6): 625-36.3. Facci, M.R.et al.(2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.Immunology. 129 (3): 396-405.4. Edamura, K.et al.(2005) Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells.Xenotransplantation. 12 (4): 327-32.5. Debeer, S.et al.(2013) Comparative histology and immunohistochemistry of porcine versus human skin.Eur J Dermatol. 23 (4): 456-66.6. Loss, H.et al.(2018) Effects of a pathogenic ETEC strain and a probioticEnterococcus faeciumstrain on the inflammasome response in porcine dendritic cells.Vet Immunol Immunopathol. 203: 78-87.7. Vreman, S.et al.(2018) Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.Dev Comp Immunol. 84: 361-70.8. LeLuduec, J.B.et al.(2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.Vaccine. 34 (7): 914-22. |
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