产品介绍 |
| Rabbit anti PAK1 (pThr84) antibodyrecognizes p21-activated kinases (PAK) 1, also known as serine/threonine-protein kinase PAK 1, alpha-PAK and p65-PAK, when phosphorylated at threonine 84.Serine/threonine-protein kinase PAK1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link the Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins are a family of serine/threonine p21-activated kinases and include PAK1-6. These proteins serve as targets for small GTP binding proteins such as OSR1.OSR1, oxidative stress response 1, is activated by osmotic stresses including sorbitol or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at threonine 84 and inhibits activation of the JNK and MAPK signaling pathway.PAK proteins also serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Bovine Expected from Sequence |
| Dog Expected from Sequence |
| Chicken Expected from Sequence |
| Guinea Pig Expected from Sequence |
| Rat Expected from Sequence |
| Sheep Expected from Sequence |
| Zebrafish Expected from Sequence |
| Mouse √ |
| Primate Expected from Sequence |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to PAK1 (pThr84) was raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity purification via sequential chromatography on phospho- and dephospho-peptide columns |
Buffer Solution | HEPES buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin50% Glycerol |
Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr84 (pThr84) region of human PAK1 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti Human PAK1 (pThr84) detects a band at approximately 68 kDa in mouse hippocampal lysate
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