产品介绍 |
| Goat anti Human AMPK alpha 2 antibodyrecognizes human5′-AMP-activated protein kinase catalytic subunit alpha-2, also known as AMPK alpha 2, ACACA kinase, Acetyl-CoA carboxylase kinase or Hydroxymethylglutaryl-CoA reductase kinase, encoded by the PRKAA2 gene. AMPK alpha 2 is a a Serine/threonine-protein kinase and the catalytic subunit of the AMP-activated protein kinase (AMPK), a central metabolic sensor, essential for the regulation of fatty acid and cholesterol synthesis, in response to fluctuations in nutrient and intracellular energy levels.AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roachet al.2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Eganet al.2011). |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Bovine Expected from Sequence |
| Dog Expected from Sequence |
| Mouse √ |
| Rat √ |
| Pig Expected from Sequence |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2). |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/16000 | |
Western Blotting | √ | 1.0 | 3.0 ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.
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二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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QQ:1749072012