Sheep anti Human carbonic anhydrase II antibody
货号：AHP206
规格：1 ml
价格：2992
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：carbonic anhydrase II
物种：人
宿主：Sheep
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Sheep anti Human carbonic anhydrase II antibodyrecognizes human carbonic anhydrase II, also known as Carbonate dehydratase II or Carbonic anhydrase C. Carbonic anhydrase II is a 259 amino acid ~30 kDa enzyme essential for bone resorption and osteoclast differrentiation.Mutations in the CA2 gene has been identified as the cause of osteopetrosis, autosomal recessive 3 (OPTB3), a rare disease characterized by particularly dense bone, cerebral calcification and renal tubular acidosis (Shahet al2004).Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography. |
Buffer Solution | Glycine buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) 0.1% EACA0.01% Benzamidine1 mM EDTA |
Immunogen | Purified human carbonic anhydrase II (CAII) prepared from erythrocytes. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| Immunodiffusion | √ | | |
| Immunofluorescence | √ | | |
| Western Blotting | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western BlottingSheep anti human carbonic anyhdrase II recognises a band of ~18kDa in rat adrenomedullary chromaffin cell lysates, seeSalmanet al.for details. |
| 文献 |
| 1. Sudarov, A.et al.(2011) Ascl1 genetics reveals insights into cerebellum local circuit assembly.J Neurosci. 31: 11055-69.2. Salman, S.et al.(2013) Chronic exposure of neonatal rat adrenomedullary chromaffin cells to opioids in vitro blunts both hypoxia and hypercapnia chemosensitivity.J Physiol. 591 (Pt 2): 515-29.3. Salman, S.et al.(2014) Chronic opioids regulate KATPchannel subunit Kir6.2 and carbonic anhydrase I and II expression in rat adrenal chromaffin cells via HIF-2α and protein kinase A.Am J Physiol Cell Physiol. 307: C266-77.4. Gailly, P.et al.(2008) A novel renal carbonic anhydrase type III plays a role in proximal tubule dysfunction.Kidney Int. 74 (1): 52-61.5. Sterling, D.et al.(2002) The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase II.Am J Physiol Cell Physiol. 283 (5): C1522-9.6. Alvarez, L.et al.(2001) Carbonic anhydrase II associated with plasma membrane in a human pancreatic duct cell line (CAPAN-1).J Histochem Cytochem. 49 (8): 1045-53.7. Alvarez, B.V.et al.(2003) Direct extracellular interaction between carbonic anhydrase IV and the human NBC1 sodium/bicarbonate co-transporter.Biochemistry. 42 (42): 12321-9.8. Sterling, D.et al.(2002) The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV.J Biol Chem. 277 (28): 25239-46.9. Long, K.L.P.et al.(2021) Regional gray matter oligodendrocyte- and myelin-related measures are associated with differential susceptibility to stress-induced behavior in rats and humans.Transl Psychiatry. 11 (1): 631. |
技术参数 产品应用 ID;IF;WB
产品类型 Polyclonal Antibody
推荐用于 ID;IF;WB
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