ANTI-HUMAN CD73 (AD2) PE-CY7
货号：25-0739-41,25-0739-42
规格：25T,100T
价格：0,3257
产品类型：流式抗体
品牌：eBioscience
抗原：CD73
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：AD2
荧光染料：PE-Cy7
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.25µg）/test(Flow） |
| Description: This AD2 monoclonal antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.Applications Reported: This AD2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AD2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD73 Antibody (25-0739-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD73 PE-Cyanine7 (purple histogram). Cells in the lymphocyte gate were used for analysis. |
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| 间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD73流式抗体细胞因子 |
| 参考文献： |
| 1.The Journal of experimental medicineAdenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression.2007Authors Deaglio S,Dwyer KM,Gao W,Friedman D,Usheva A,Erat A,Chen JF,Enjyoji K,Linden J,Oukka M,Kuchroo VK,Strom TB,Robson SC2.Journal of immunology (Baltimore, Md. : 1950)T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5'-adenosine monophosphate to adenosine.2006Authors Kobie JJ,Shah PR,Yang L,Rebhahn JA,Fowell DJ,Mosmann TR |
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