Rabbit anti Human TIMP-1:Biotin
货号：AHP2187B
规格：50 µg
价格：4208
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：TIMP-1
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Biotin
产品介绍 |
| Rabbit anti Human TIMP-1 antibodyrecognizes human TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. A correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth (Sternlicht and Werb 2001) |
产品详情 |
Target Species | Human |
Product Form | Purified IgG conjugated to Biotin - lyophilized |
Reconstitution | Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
AntiserumPreparation | Antiserum to human TIMP-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Carrier Free | Yes |
Immunogen | Recombinant human TIMP-1. |
Approx. Protein Concentrations | IgG concentration 0.1mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.25 | 1.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISA
- This biotinylated human TIMP-1 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with ourpurified human TIMP-1 antibody(AHP2187) as the capture reagent.
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技术参数 产品应用 E；WB
产品类型 Polyclonal Antibody
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