Mouse anti Human Collagen II
货号：2150-0040
规格：0.1 ml
价格：7013
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Collagen II
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：COLL-II
荧光染料：其它
| 产品介绍 | | |
| Mouse anti Collagen II antibody, clone COLL-IIrecognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX. |
| 产品详情 | | |
Target Species | Human |
Product Form | Ascites - liquid |
Preservative Stabilisers | None present |
Immunogen | Human (cartilage specific) CNBr-cleaved collagen 2. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/100 | 1/500 |
Immunofluorescence1 | √ | Neat | 1/5 |
| Immunohistology - Paraffin | √ | | 1/50 |
Western Blotting | √ | 1/1000 | 1/2000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 数据 |
Mouse anti Human collagen II antibody, clone COLL-II (2150-0040) used to demonstrate collagen II staining on human colon by immunohistochemistry with formalin fixed, paraffin embedded tissue sections. |
| 文献 |
1. Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.Br J Haematol. 90 (4): 757-66.2. Herrero-mendez, A.et al.(2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.J Tissue Eng Regen Med. 11 (4): 989-1001.3. Glant, T.T.et al.(1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.Histochemistry. 82 (2): 149-58.4. Hashemi Beni, B.et al.(2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture SystemIranian J basic Med Res. 11: 10-17
Further Reading1.Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.Biochemistry. 11 (26): 4903-9. |
技术参数 产品应用 ELISA;IF*;IHC(P);WB
产品类型 Monoclonal Antibody
推荐用于 ELISA;IF*;IHC(P);WB
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