Goat anti Human CD137: Biotin
货号：AHP1863B
规格：50 µg
价格：4208
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：CD137
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Biotin
| 产品介绍 | | |
| Goat anti Human CD137 antibodyspecifically recognizes human CD137, otherwise known as 4-1BB ligand receptor, a type I transmembrane glycoprotein and member of the tumour necrosis factor receptor superfamily, predominantly expressed by activated T cells, which acts as a receptor for 4-1BB ligand (4-1BBL).Interaction between CD137 and 4-1BBL, acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. CD137 also binds to the adaptor proteins TRAF1, 2 and 3 to act as a signal transducer of NF-kappaB activation. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Biotin - lyophilized |
| Reconstitution | Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
Antiserum Preparation | Antiserum to human CD137 were raised by repeated immunisation of goats with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
Immunogen | Recombinant human CD137 |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
| Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
技术参数 产品应用 E;WB
产品类型 Polyclonal Antibody
推荐用于 E;WB
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