| 产品介绍 | | |
| Rabbit anti Human Bik antibodyrecognizes the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk). Bik is a Bcl-2 homology domain 3 (BH3)-only protein of ~18 kDa which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis.Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to Bik were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | 15 amino acid peptide from near the amino terminus of human Bik. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | |
Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1172 detects a band of approximately 18kDa in Jurkat cell lysates.
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
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| 数据 |
Immunocytochemical staining of Jurkat cells with Rabbit anti Human BIK (AHP1172). |
| 文献 |
| 1. Mathai, J.P.et al.(2005) BH3-only BIK regulates BAX,BAK-dependent release of Ca2+ from endoplasmic reticulum stores and mitochondrial apoptosis during stress-induced cell death.J Biol Chem. 280 (25): 23829-36.2. Real, P.J.et al.(2006) Transcriptional activation of the proapoptotic bik gene by E2F proteins in cancer cells.FEBS Lett. 580 (25): 5905-9.3. Sturm, I.et al.(2006) Loss of the tissue-specific proapoptotic BH3-only protein Nbk/Bik is a unifying feature of renal cell carcinoma.Cell Death Differ. 13 (4): 619-27. |
QQ:1749072012