产品介绍 |
| Goat anti Human CD274 antibodyrecognizes an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons.CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1. |
产品详情 |
Target Species | Human |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1). |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/8000 | |
Immunohistology - Paraffin1 | √ | 4.0 | 6.0ug/ml |
Western Blotting | √ | 0.3 | 1.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human skeletal muscle
- Western Blotting
- AHP2128 detects a band of approximately 35kDa in human heart cell lysates.
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可用二抗 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
相关产品 |
Description | Product Code | Applications | Pack Size |
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml |
文献 |
| 1.Duchnowska, R.et al.(2016) Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.Breast Cancer Res. 18 (1): 43. |
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