Goat anti Human p70S6K (C-Terminal)
货号：AHP1575
规格：0.1 mg
价格：5083
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：p70S6K (C-Terminal)
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti phospho-70S6K antibodyrecognizes an epitope within the C-terminal (CT) region of human p70S6K, otherwise known as RPS6KB1 or Ribosomal protein S6 kinase beta-1. 70S6K is a serine/threonine kinase which acts upon 40S ribosomal protein S6 to induce protein synthesis, allowing for efficient G1 phase progression.p70S6K is activated following phosphorylation of several sites, in response to hormones, growth factors, mitogenic stimuli and physical exercise, and is a downstream effector in the AKT/mTOR (mammalian target of rapamycin) signalling pathway. p70S6K also acts as a regulator of cell motility, invasion, and cytoskeletal reorganization. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human p70S6K (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide sequence C-MISKRPEHLRMNL corresponding to the C-Terminal region of p70S6K (NP_003152.1). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/128000 |
Western Blotting | √ | 0.2 | 1.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1575 detects a band of approximately 65-70kDa in human muscle and placenta cell lysates.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
Immunohistochemical staining of paraffin embedded human kidney following heat mediated antigen retrieval with citrate buffer at pH 6.0 using Goat anti p70S6K (AHP1575) at 2.0μgml-1, followed by anti Goat - HRP staining and DAB chromogen development |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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