Rabbit anti 5-lipoxygenase (pSer523) antibody
货号：AHP1538
规格：0.1 ml
价格：6095
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：5-lipoxygenase (pSer523)
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti 5-lipoxygenase (pSer523) antibodyrecognizes 5-lipoxygenase (5-LO), also known as arachidonate 5-lipoxygenase, when phosphorylated at serine 523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LTs) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LTs include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to phosphorylated 5-lipoxygenase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | 10mM HEPES pH7.5, 150mM NaCl |
Preservative Stabilisers | 0.09%Sodium Azide 0.01%Bovine Serum Albumin 50%Glycerol |
Immunogen | Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes the phosphorylated residue Ser523. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1538 detects a doublet of approximately 80kDa in rat cortex lysate.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Western blot of rat hippocampal lysate showing phospho-specific immunolabelling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523using Rabbit anti 5-lipoxygenase (pSer523) antibody (AHP1538). |
技术参数 产品应用 WB
产品类型 Polyclonal Antibody
推荐用于 WB
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-35035.htm
QQ:1749072012