CD16/CD32 Monoclonal Antibody (93)/ FcR blocker/FcR阻断剂

货号：14-0161-81,14-0161-82,14-0161-85,14-0161-86

规格：50μg,100μg,500μg,1mg

价格：717,1168,2712,4835

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：93

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.5 µg/test(Flow)；Assay-Dependent (FN)

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis. 93 has also been reported in blocking of Fc-mediated reactions in functional studies. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with 0.5-1 µg of purified anti-CD16/CD32 per million cells for 5-10 minutes on ice prior to staining. (Please use Functional Grade purified 93, cat. 16-0161, in functional assays.).Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD16/CD32 Antibody (14-0161-81) in Flow Staining of BALB/c splenocytes with 0.25 µg Purified Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg Anti-Mouse CD16/32 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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参考文献：

1. Nature communicationsDiabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages."14-0161 was used in Flow cytometry/Cell sorting to demonstrate for the first time that type 2 diabetes mellitus induces an haematopoietic stem cell-autonomous mechanism that causes impaired wound healing."AuthorsYan J,Tie G,Wang S,Tutto A,DeMarco N,Khair L,Fazzio TG,Messina LM2. The EMBO journalThe WD40 domain of ATG16L1 is required for itsnon-canonical role in lipidation of LC3 at singlemembranes."14-0161 was used in Flow cytometry/Cell sorting to demonstrate activation of non-canonical autophagy dependent on the WD40 CTD during influenza A virus infection."AuthorsFletcher K,Ulferts R,Jacquin E,Veith T,Gammoh N,Arasteh JM,Mayer U,Carding SR,Wileman T,Beale R,Florey O3. Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. NatureMyeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5．OncoimmunologyVectorization in an oncolytic vaccinia virus of an antibody, a Fab and a scFv against programmed cell death -1 (PD-1) allows their intratumoral delivery and an improved tumor-growth inhibition."14-0161 was used in Flow cytometry/Cell sorting to develop a method to vectorise a hamster monoclonal IgG against murine PD-1 in Western Reserve oncolytic vaccina virus."AuthorsKleinpeter P,Fend L,Thioudellet C,Geist M,Sfrontato N,Koerper V,Fahrner C,Schmitt D,Gantzer M,Remy-Ziller C,Brandely R,Villeval D,Rittner K,Silvestre N,Erbs P,Zitvogel L,Quéméneur E,Préville X,Marchand JB

技术参数

产品应用 Flow

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