Rabbit anti Human WNT-1 antibody
货号：AHP1287
规格：0.1 mg
价格：4063
产品类型：免疫组化一抗
品牌：Bio-Rad
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human WNT-1 antibodyrecognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor-like virus (Wainwright 1988). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended. |
| Antiserum Preparation | Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present |
| Immunogen | Recombinant human Wnt-1. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
| Regulatory | For research purposes only |
| Guarantee | 6 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2.0ug/ml |
| Functional Assays | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Brown, A.M.et al.(1987) Identification of protein products encoded by the proto-oncogene int-1.Mol Cell Biol. 7 (11): 3971-7. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
推荐用于 elisa；wb
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