FOXO3A Polyclonal Antibody/FOXO3A多克隆抗体

货号：720128

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to Human FOXO3 (aa 29-37)

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-2 µg x 10^6 cells(ChIP)；1-2 µg/mL(ICC)；1-2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.This antibody is predicted to react with Monkey, Goat, Pig, Bovine and Rabbit.靶标信息USP48 is a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.

数据：

FOXO3A Antibody (720128) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of FOXO3A using Anti-FOXO3A Rabbit Polyclonal Antibody (Product # 720128, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of FOXO3A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of FOXO3A. Panel e) represents control cells with no primary Antibody to assess background. FOXO3A Antibody (720128) in ChIPChromatin Immunoprecipiation (ChIP) was performed using Anti-FOXO3a Rabbit Polyclonal Antibody (Product # 720128, 3 µg) on sheared chromatin from 2 million Jurkat cells serum starved for 24 hours using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active BCL10 region used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method. FOXO3A Antibody (720128) in WBWestern blot analysis was performed on whole cell extracts (50 µg lysate) of HEL (Lane1), Mouse Placenta (Lane 2) and Mouse Liver (Lane 3). The blots were probed with Anti-FOXO3A Rabbit Polyclonal Antibody (Product # 720128, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 71 kDa band corresponding FOXO3A was observed in cell line and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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