Goat anti Human TNF alpha antibody
货号：AHP1212
规格：0.1 mg
价格：4063
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：TNF alpha
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Human TNF alpha antibodyrecognizes human Tumour Necrosis Factor alpha (TNF–alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide. |
| Antiserum Preparation | Antisera to human TNFα were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present. |
| Immunogen | Recombinant human TNFα |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
| Regulatory | For research purposes only |
| Guarantee | 6 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.25 | 1.0ug/ml |
| Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 100 Tests |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C,E,WB | 100 Tests |
| 文献 |
| 1.van Horssen, R.et al. (2006) TNF-α in cancer treatment: Molecular insights, antitumor effects, and clinical utility.Oncologist. 11: 397-408. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
推荐用于 elisa；wb
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