Rabbit anti Human interleukin-32 antibody
货号：AHP1206
规格：0.1 mg
价格：2933
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：interleukin-32
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human interleukin-32 antibodyrecognises IL-32, a ~26 kDa cytokine, thought to play a role in innate and adaptive immune responses. IL-32 is produced by mitogen-activated lymphocytes, interferon-gamma activated epithelial cells and IL-12, IL-18, IL-32-activated NK cells. It is expressed at elevated levels after the activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-kappa-B and p38 MAPK cytokine signal pathways. TNF-alpha, IL-1beta, IL-6 and 2 C-X-C chemokine family members, all involved in autoimmune and inflammatory diseases, including rheumatoid arthritis are induced by IL-32. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preparation | Antisera to human IL-32 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Immunogen | A 16 amino acid peptide located near the centre of human IL-32. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 5 | 10ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Conti, P. (2007) Modulation of autoimmunity by the latest interleukins (with special emphasis on IL-32).Autoimmun Rev. 6:131-7.2.Chen, Q. (2006) The newest interleukins: recent additions to the ever-growing cytokine family.Vitam Horm. 74:207-28.3.Kim, S. (2005) Interleukin-32: a cytokine and inducer of TNFalpha.Immunity. 22:131-42.4.Cagnard, N. (2005) Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes.Eur Cytokine Netw. 16:289-92. |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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