| 产品介绍 | | |
| Goat anti Syk antibodydetects an epitope within the internal region of SYK, a ~70 kDa non-receptor protein tyrosine kinase whose role differs in haematopoietic and non haematopoietic stem cells. In haematopoietic cells SYK is involved in the proximal signalling downstream of activated immunoreceptors (BCR, TCR, Fc receptors), thereby orchestrating a complex series of cellular responses such as cell proliferation, differentiation, survival and phagocytosis.In epithelial cells SYK appears to function as a negative regulator of related events. Furthermore, SYK has been shown to promote expression or secretion of inflammation-related molecules in lung epithelial cells.SYK appears to be required for vascular integrity during development, thus, manipulation of SYK function in endothelial cells could potentially modulate tumour-associated angiogenesis. SYK also has been shown to interact with Epstein-Barr virus LMP2A. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to SYK were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) 0.5%Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide with sequence C-KYLQQNRHVKDKN from the internal region of the SYK sequence. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/16000 |
Western Blotting | √ | 1.0 | 3.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1066 detects a band of approximately 70kDa in human bone marrow, lymph node and spleen lysates.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
| Western blotting analysis of Human spleen lysate (35μg protein in RIPA buffer) using Goat anti Human SYK antibody (AHP1066) at 1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence. |
| 文献 |
| 1. Coopman, P.J. & Mueller, S.C. (2006) The Syk tyrosine kinase: a new negative regulator in tumor growth and progression.Cancer Lett. 241 (2): 159-73.2. Wong, B.R.et al.(2004) Targeting Syk as a treatment for allergic and autoimmune disorders.Expert Opin Investig Drugs. 13 (7): 743-62. |
QQ:1749072012