| 产品介绍 | | |
| Goat anti Human Osteopontin antibodyrecognises an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated ~60 kDa glycoprotein. Osteopontin is a secreted chemokine-like protein and member of the SIBLING family, and is synthesised by many cell types.Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid, ready to use. |
Antiserum Preparation | Antisera to osteopontin (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) 0.5%Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/ 64000 |
Western Blotting | √ | 1.0 | 3.0 ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1069 detects a band of approximately 60kDa in lysates of human thymus, tonsil and spleen. Approximate 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glcoprotein.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
| Western blotting analysis of MOLT4 lysate (35µg protein in RIPA buffer) using Goat anti Human osteopontin antibody (AHP1069) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence. |
| 文献 |
| 1. Rangaswami, H.et al. (2006) Osteopontin: role in cell signaling and cancer progression.Trens Cell Biol. 16: 79-87. |
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