| 产品介绍 | | |
| Goat anti Human GAPDH antibodyrecognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Dastooret al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burkeet al.1996). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Dog | √ |
| Mouse | √ |
| Pig | √ |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human GAPDH (CT) were raised by repeatedimmunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) 0.5%Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/2000 |
Immunohistology - Paraffin1 | √ | | 0.3ug/ml |
Western Blotting | √ | 0.01 | 0.03ug/ml |
1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human tonsil
- Western Blotting
- AHP1064 detects a band of approximately 35kDa in human tonsil, mouse spleen and HeLa cell lysates.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
Western blotting analysis of mouse liver (A) and rat brain (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence. |
| 文献 |
| 1. Hara, M.R.et al.(2006) GAPDH as a sensor of NO stress.Biochim Biophys Acta. 1762 (5): 502-9.2. Mazzola, J.L. & Sirover, M.A. (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.J Neurochem. 76 (2): 442-9.3. Wang Het al.(2013) Hypoxic preconditioning alleviates ethanol neurotoxicity: the involvement of autophagy.Neurotox Res. 24 (4): 472-7. |
QQ:1749072012