5’-DMT-T(C6 Amino)-Succinate Super Column (5'-DMT-T(Hexyl-NH-TFA)-Suc-CPG); 500 Å
货号:SCG5-5009-1,SCG5-5009-2
规格:1 µmol,200 nmol
价格:562.8,211.05
产品类型:核酸合成
品牌:LGC Biosearch Technologies
特性: |
▪ Appearance:white powder▪ Extinction Coefficient at 260 nm:8400 |
产品用途: |
Cleavage conditions:Before cleavage and deprotection treat the oligo attached to the support with 10% DEA (diethylamine) in MeCN for 5-10 minutes to avoid acrylonitrile addition to the amine during cleavage and deprotection. After treatment rinse the support with MeCN, then continue to deprotect and cleave.
Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.The 10% DEA (diethylamine) in MeCN treatment of the oligo while still on support removes the acrylonitrile that is produced from the beta elimination of the cyanoethyl groups during deprotection. The acrylonitrile can attach to the amine generated after the removal of the TFA group during the cleavage and deprotection cycle. A +53 adduct will be seen on the mass spec of the oligo if acrylonitrile addition has occurred. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:378.44 |
数据: |
|
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://biorod.cn/thread-21047.htm