产品介绍 | | |
Rabbit anti Rat Interleukin-1 beta antibodyrecognizes rat IL-1 beta. Interleukin-1β is a 152 amino acid active pro-inflammatory cytokine produced with an additional 116 amino acid pro-peptide region. IL-1&beta has a broad mode of action, stimulating prostaglandin synthesis, neutrophil, T cell and B cell activation and collagen synthesis. |
产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to rat IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution. |
Immunogen | Recombinant rat IL-1 beta (PRP23). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
存储条件 | | |
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5ug/ml | 2.0ug/ml |
Functional Assays | √ | 0.09ug/ml | 0.14ug/ml |
Immunohistology - Paraffin | √ | | |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAThis product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together withAAR15Bas the detection reagent andPRP23as the standard. |
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文献 |
1. Girard, S. (2008) Pro-inflammatory disequilibrium of the IL-1 beta/IL-1ra ratio in an experimental model of perinatal brain damages induced by lipopolysaccharide and hypoxia-ischemia.Cytokine. 43: 54-62.2. Glatz, T.et al.(2010) Peroxisome-proliferator-activated receptors gamma and peroxisome-proliferator-activated receptors beta/delta and the regulation of interleukin 1 receptor antagonist expression by pioglitazone in ischaemic brain.J Hypertens. 28: 1488-97.3. Mahmood, J.et al.(2011) Mitigation of radiation-induced lung injury by genistein and EUK-207.Int J Radiat Biol. 87: 889-901.4. Weksler-Zangen, S.et al.(2008) Impaired glucose-stimulated insulin secretion is coupled with exocrine pancreatic lesions in the Cohen diabetic rat.Diabetes. 57: 279-87.5. Mahmood, J.et al.(2013) Mitigation of radiation-induced lung injury with EUK-207 and genistein: effects in adolescent rats.Radiat Res. 179 (2): 125-34. |