| 产品介绍 | | |
| Rabbit anti Mouse VEGF antibodyrecognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth.Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution. |
Immunogen | Recombinant mouse VEGF |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 0.5ug/ml |
Functional Assays | √ | 1.0ug/ml | 2.0ug/ml |
| Immunofluorescence | √ | 2.5ug/ml | |
| Immunohistology - Paraffin1 | √ | 2.5ug/ml | |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- ELISA
- This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA withAAM51Bas a detection reagent andPMP67as the standard.
- Histology Positive Control Tissue
- Kidney
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| 文献 |
| 1. Nahrendorf, M.et al.(2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.J Exp Med. 204: 3037-47.2. Araújo, W.F.et al.(2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.Urol Oncol. 33 (9): 389.e1-7.3. Lin, E.Y.et al.(2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.Mol Oncol. 1: 288-302.4. HogenEsch Het al.(2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.Exp Mol Pathol. Oct 26. pii: S0014-4800(16)30084-3. [Epub ahead of print]5. Hogenesch, H.et al.(2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.Exp Mol Pathol. 101 (3): 303-7. |
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