Goat anti Human RAB8A (C-Terminal)
货号：AHP1683
规格：0.1 mg
价格：5083
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原： RAB8A
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
产品介绍 |
| Goat anti Human RAB8A antibodyrecognizes an epitope within the C-terminal (CT) region of the human RAS superfamily member Rab8a (Ras-related protein Rab-8A), otherwise known as oncogene c-mel, Rab8A is a small monomeric GTP/GDP-binding protein with a predicted molecular weight of ~24 kDa which may play a role in vesicular trafficking and neurotransmitter release.The Rab family of proteins, originally described asRas-like proteins inbrain are the largest group of GTPases within the Ras superfamily with 61 protein members identified. Rab family members are involved in the regulation of intracellular vesicular transport and movement of proteins between endocytic and secretory organelles (Wennerberget al.2005). Rab8A is involved in vesicular traffic between the trans golgi network and the basoleteral aspect of the plasma membrane (Huberet al.1993).Goat anti human Rab8a antibody has been successfully used for the detection of Rab8a in human intestinal epithelial cells using western blotting (Vaccaet al.2014). |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target SpeciesCross Reactivity |
| Dog Expected from Sequence |
| Bovine Expected from Sequence |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human RAB8A (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Peptide sequence C-NQGVKITPDQQKR from the C-Terminal region of RAB8A (NP_005361.2). |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/32000 |
Western Blotting | √ | 0.1 | 0.3ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1683 detects a band of approximately 25kDa in human cerebellum and kidney cell lysates
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相关产品 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
文献 |
1.Vacca, B.et al.(2014) Drebrin E depletion in human intestinal epithelial cells mimics Rab8a loss of function. Hum Mol Genet. 23 (11): 2834-46. |
技术参数 产品应用 E;WB
产品类型 Polyclonal Antibody
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