IL-10 Monoclonal Antibody (JES5-16E3), APC

货号：17-7101-82,17-7101-81

规格：100μg,50μg

价格：2788,1674

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：JES5-16E3

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.125µg/test(Flow）

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.Applications Tested: The JES5-16E3 antibody has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationInterleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

推荐产品：

流式抗体细胞周期检测细胞凋亡检测细胞增殖活性检测

参考文献：

1. Oncoimmunologymyeloid-derived suppressor cells as a readout of therapeutic efficacy."17-7101 was used in Flow cytometry/Cell sorting to show that the simultaneous delivery of IL12 and a PD-L1-silencing shRNA exhibits therapeutically relevant anti-tumour activities."AuthorsLiechtenstein T,Perez-Janices N,Blanco-Luquin I,Goyvaerts C,Schwarze J,Dufait I,Lanna A,Ridder M,Guerrero-Setas D,Breckpot K,Escors D2. PloS oneMHC-I and PirB Upregulation in the Central and Peripheral Nervous System following Sciatic Nerve Injury."17710182 was used in flow cytometry to measure expression of major histocompatibility complex class one and paired immunoglobulin-like receptor B in the central and peripheral nervous system after sciatic nerve injury in mice"AuthorsBombeiro AL,Thomé R,Oliveira Nunes SL,Monteiro Moreira B,Verinaud L,Oliveira AL3. Scientific reportsLymphocytic Microparticles Modulate Angiogenic Properties of Macrophages in Laser-induced Choroidal Neovascularization."17710182 was used in flow cytometry to study the ability of lymphocyte-derived microparticles to modulate the gene expression pattern of angiogenesis-related factors in macrophages"AuthorsTahiri H,Omri S,Yang C,Duhamel F,Samarani S,Ahmad A,Vezina M,Bussières M,Vaucher E,Sapieha P,Hickson G,Hammamji K,Lapointe R,Rodier F,Tremblay S,Royal I,Cailhier JF,Chemtob S,Hardy P4. PLoS pathogensCytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence."17-7101 was used in Flow cytometry/Cell sorting to reveal that cytokine signalling events during initial infection profoundly influence virus chronicity."AuthorsClement M,Marsden M,Stacey MA,Abdul-Karim J,Gimeno Brias S,Costa Bento D,Scurr MJ,Ghazal P,Weaver CT,Carlesso G,Clare S,Jones SA,Godkin A,Jones GW,Humphreys IR5. Neurology(R) neuroimmunology and neuroinflammationCNS accumulation of regulatory B cells is VLA-4-dependent."17-7101 was used in Immunocytochemistry to demonstrate that accumulation of Breg in the CNS is dependent on VLA-4."AuthorsLehmann-Horn K,Sagan SA,Winger RC,Spencer CM,Bernard CC,Sobel RA,Zamvil SS

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17047.htm