| 产品介绍 | | |
| Goat anti Dog IgA polyclonal antibodyrecognizes canine IgA and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA.Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiacket al.2013) and with mustelids (Martelet al.2009). |
| 产品详情 | | |
Target Species | Dog |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mustelid | Expected from Sequence |
| Ferret | √ |
| Wolf | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid |
| Antiserum Preparation | Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.05% Proclin 3000.2% Bovine Serum Albumin |
Immunogen | Purified canine IgA. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/10000 | 1/80000 |
| Immunohistology - Frozen | √ | 1/200 | 1/500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Frankowiack, M.et al.(2013) IgA deficiency in wolves.Dev Comp Immunol. 40 (2): 180-4.2. Olsson Met al.(2014) The dog as a genetic model for immunoglobulin A (IgA) deficiency: identification of several breeds with low serum IgA concentrations.Vet Immunol Immunopathol. 160 (3-4): 255-9.3. Frankowiack, M.et al.(2015) IgA deficiency in wolves from Canada and Scandinavia.Dev Comp Immunol. 50 (1): 26-8.4. Lee, A.et al.(2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.J Comp Pathol. 152 (2-3): 201-5.5. Tengvall, K.et al.(2013) Genome-wide analysis in German shepherd dogs reveals association of a locus on CFA 27 with atopic dermatitis.PLoS Genet. 9 (5): e1003475.6. Martínez-López, L.M.et al.(2021) Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.PLoS One. 16 (8): e0255012.7.Sfacteria, A.et al.(2021) Immune Cells and Immunoglobulin Expression in the Mammary Gland Tumors of Dog.Animals (Basel). 11(5):1189.8. Rossi, G.et al.(2020) Effects of the Probiotic Mixture Slab51®(SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.Front Vet Sci. 7: 613.9. Satyaraj, E.et al.(2021) Supplementation of Diets WithSpirulinaInfluences Immune and Gut Function in Dogs.Front Nutr. 8: 667072. |
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