产品介绍 | | |
Goat anti Chicken IgM antibodyrecognizes chicken immunoglobulin M and shows no cross reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.Goat anti chicken IgM antibody may cross react with IgM from other species. |
产品详情 | | |
Target Species | Chicken |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to feline IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Immunogen | Purified bovine IgM. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
存储条件 | | |
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/100 | 1/1000 |
Immunohistology - Frozen | √ | 1/200 | 1/2000 |
Immunocytochemistry | √ | 1/200 | 1/2000 |
Immunodiffusion | √ | | |
Western Blotting | √ | 1/1000 | 1/30000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
文献 |
1. Withanage, G.S.et al.(2001) Cytokine and chemokine responses associated with clearance of a primarySalmonella entericaserovarTyphimuriuminfection in the chicken and in protective immunity to rechallenge.Infect Immun.73: 5173-82.2. Beal, R.K.et al.(2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.Infect Immun. 73 (11): 7509-16.3. Rautenschlein, S.et al.(2011) Local and systemic immune responses following infection of broiler-type chickens with avian Metapneumovirus subtypes A and B.Vet Immunol Immunopathol. 140(1-2):10-22.4. Toscano, M.J.et al.(2010) Sub-clinical infection withSalmonellain chickens differentially affects behaviour and welfare in three inbred strains.Br Poult Sci. 51: 703-13.5. Radomska, K.A.et al.(2016) Chicken Immune Response afterIn OvoImmunization with Chimeric TLR5 Activating Flagellin ofCampylobacter jejuni.PLoS One. 11 (10): e0164837.6. Dziewulska, D.et al.(2018) The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.BMC Vet Res. 14 (1): 148.7. Konieczka, P.et al.(2022) Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.Vet Res. 53 (1): 59. |