Rabbit anti Porcine tubulin antibody
货号：9280-0050G
规格：0.5 ml
价格：2236
产品类型：免疫组化一抗
品牌：Bio-Rad
物种：其它
宿主：兔
抗体亚型：IgG3
荧光染料：Alexa Fluor 488
| 产品介绍 | | |
| Rabbit anti Porcine tubulin antibodyrecognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution.Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions. |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide (NaN3) |
| Immunogen | Purified porcine brain tubulin. |
| Approx. Protein Concentrations | IgG concentration 5.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/50 | 1/500 |
| Western Blotting | √ | 1/250 | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Gelfand, V.I. & Bershadsky, A.D. (1991) Microtubule dynamics: mechanism, regulation, and function.Annu Rev Cell Biol. 7: 93-116.2.Downing, K.H. & Nogales, E. (1998) Tubulin structure: insights into microtubule properties and functions.Curr Opin Struct Biol. 8 (6): 785-91.3.Wang, M. & Collins, R.N. (2014) A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint.Mol Biol Cell. 25 (18): 2720-34.4.Coluccio, L.M.et al.(2021) Enhancing Antibodies’ Binding Capacity through Oriented Functionalization of Plasmonic SurfacesNanomaterials. 11 (10): 2620. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
推荐用于 elisa；wb
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