| 产品介绍 | | |
| Sheep anti bovine IgMrecognizes bovine IgM and shows no cross-reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. Sheep anti bovine IgM may cross-react with IgM from other species. |
| 产品详情 | | |
Target Species | Bovine |
Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antisera to bovine IgM were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide |
Immunogen | Purified bovine IgM. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/100 | 1/1000 |
| Immunodiffusion | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Nebl, T.et al.(2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.J Biol Chem. 277 (45): 43399-409.2. Assad, A.et al.(2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.Vet Immunol Immunopathol. 147: 25-34.3. Hamsten, C.et al.(2009) Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected byMycoplasma mycoidessubsp.mycoidessmall colony type.Mol Cell Proteomics. 8: 2544-54.4. Mansilla FCet al.(2015) Safety and immunogenicity of a soluble nativeNeospora caninumtachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in pregnant cattle.Vet Immunol Immunopathol. 165 (1-2): 75-80.5. Hossain, M.M.et al.(2016) Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine.Vector Borne Zoonotic Dis. Jul 5. [Epub ahead of print]6. Van Meulder, F.et al.(2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasiteCooperia oncophora.Int J Parasitol. 45 (9-10): 637-46.7. Jankowska, A.et al.(2016) Humoral and cellular immune response toHistophilus somnirecombinant heat shock protein 60 kDa in farm animalsVeterinární Medicína. 60 (No. 11): 603-12.8. Çokçalışkan, C.et al.(2019) Effect of simultaneous administration of foot-and-mouth disease (FMD) and anthrax vaccines on antibody response to FMD in sheep.Clin Exp Vaccine Res. 8 (2): 103-9.9. Springer, A.et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine LungwormDictyocaulus viviparus..Pathogens 2022, 11, 55.10. Di Giacomo, Set al.(2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in CattleViruses. 14 (8): 1781. |
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