Mouse anti transferrin antibody, clone HTF-14
货号：9100-1055
规格：0.2 mg
价格：3043
产品类型：免疫组化一抗
品牌：Bio-Rad
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：HTF-14
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti transferrin antibody, clone HTF-14recognizes the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Masonet al.2009)Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Purified porcine transferrin. |
| Approx. Protein Concentrations | IgG concentration 1mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| Functional Assays | √ | | |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
| 文献 |
| 1.Rubikaite, B.I.et al.(1989) [Identification of the segment for binding of transferrin using a cellular receptor].Mol Biol (Mosk). 23: 765-71.2.Hradilek, A. and Neuwirt, J. (1986) Iron uptake by MOLT 3 cells from transferrin/monoclonal antitransferrin antibody complexes.Br J Haematol. 62: 21-30.3.Trebichavsky, I.et al.(1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.Folia Microbiol (Praha). 32: 448-52.4.Fuchs, O.et al.(1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.Biochim Biophys Acta. 969: 158-65.5.Bártek, J.et al.(1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.Br J Haematol. 59: 435-41.6.Penhallow, R.C.et al.(1986) Comparative studies of the binding and growth-supportive ability of mammalian transferrins in human cells.J Cell Physiol. 128: 251-60. |
技术参数 产品应用 elisa；wb
产品类型 Monoclonal Antibody
推荐用于 elisa；wb
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