| 产品介绍 | | |
| Rat anti substance P antibody, clone NC1/34recognizes the COOH terminal region of substance P, an eleven amino acid polypeptide neurotransmitter derived by proteolytic cleavage of protachykinin-1. Substance P binds to and activates the neurokinin-1 receptor. Substance P is a highly conserved neurotransmitter expressed throughout the animal kingdom. It is highly expressed in the spinal cord dorsal horn and dorsal root ganglia, but not in the ventral horn (Ribeiro-da-Silva andT Hökfelt 2000). It is also expressed in non-neuronal cells including microglia and other immune cells.Substance P has a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction (Hökfelt 2004).Rat anti substance P antibody, clone NC1/34 demonstrates ~5% reactivity with eledoisin a neurotransmitter identified in the octopus GenusEledone. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin in tested applications. |
| 产品详情 | | |
Product Form | Tissue Culture Supernatant - liquid |
Preservative Stabilisers | 0.05%Thiomersal |
Fusion Partners | Spleen cells from immunised Wistar rat were fused with cells of the NS1/1-Ag 4-1 myeloma cell line. |
Immunogen | Substance P conjugated to Bovine Serum Albumin. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | | |
Immunohistology - Frozen | √ | 1/100 | 1/200 |
Immunohistology - Paraffin1 | √ | 1/100 | 1/200 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product does not require antigen retrieval prior to staining of formalin fixed, paraffin embedded sections.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 二抗相关产品 |
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| 数据 |
| Published customer image:Rat anti substance P antibody, clone NC1/34 (8450-0505) used for the detection of substance P expressing cells in human tendon tissue biopsies by immunofluorescence.Image caption:Double stainings for PARs and substance P. A-D shows PARs [1]-[4] and E-H shows corresponding SP-staining; PAR-1 (A) is co-localised with SP-positive nerve fibres (E) (arrows). The same was seen for PAR-2 (nerve fascicle in B&F), PAR-3 (nerve fascicle in C&G) and PAR-4 (D&H, arrows show perivascular nerve fibres).From: Christensen, J. Alfredson, H. and Andersson, G. "Protease-activated receptors in the Achilles tendon–a potential explanation for the excessive pain signalling in tendinopathy".Mol Pain. 2015 Mar 17;11:13.This is from an open access article distributed under the terms of theCreative Commons Attribution License. |
| 文献 |
| 1. Kozsurek, M.et al.(2009) Nonselective innervation of lamina I projection neurons by cocaine- and amphetamine-regulated transcript peptide (CART)-immunoreactive fibres in the rat spinal dorsal horn.Eur J Neurosci. 29: 2375-87.2. Cuello ACet al.(1980) Use of monoclonal antibodies in immunocytochemistry with special reference to the central nervous system.Brain Res Bull. 5 (5): 575-87.3. Pioro, E.P.et al. (1985) Loss of substance P immunoreactivity in the nucleus of the spinal trigeminal tract after intradural tumour compression of the trigeminal nerve.Neurosci Lett. 58: 7-12.4. Mai, J.K.et al.(1986) Substance P in the human brain.Neuroscience. 17 (3): 709-39.5. Cuello, A.C.et al.(1979) Detection of substance P in the central nervous system by a monoclonal antibody.Proc Natl Acad Sci U S A. 76 (7): 3532-6. |
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