| 产品介绍 | | |
| Rat anti serotonin antibody, clone YC5/45recognizes serotonin and does not cross react with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.Serotonin, also known as 5-hydroxytryptamine or 5-HT is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotonin is widely distributed in the central and peripheral nervous system, and also in some non-neural tissues such as platelets.Serotonin appears widely conserved and Rat anti serotonin antibody, clone YC5/45 has been used successfully to demonstrate serotonin immunoreactivity using immunohistochemistry in many diverse groups including primates (Vanoliet al.2013), rodents (Hosodaet al.1984), prototherian mammals, avians and reptiles (Adamson and Campbell 1988), teleosts (Takedaet al.2014) through to invertebrates including hirudinids (Masuda-Nakagawaet al.1990) and gastropods (Osborne and Dockray 1982). |
| 产品详情 | | |
Target Species | Broad |
Product Form | Tissue Culture Supernatant - liquid |
Fusion Partners | Spleen cells from immunised COB wistar rats were fused with cells of the Y3-Ag-1.2.3 rat myeloma cell line. |
Preservative Stabilisers | 0.05%Thiomersal |
Immunogen | Serotonin conjugated to bovine serum albumin. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | | |
Immunohistology - Frozen1 | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Formaldehyde fixation is recommended.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
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| 文献 |
| 1. Consolazione, A.et al.(1981) Immunocytochemical detection of serotonin with monoclonal antibodies.J Histochem Cytochem. 29: 1425-30.2. Milstein, C.et al.(1983) The discrepancy between the cross-reactivity of a monoclonal antibody to serotonin and its immunohistochemical specificity.Mol Immunol. 20 (1): 113-23.3. Suárez-Quian, C.A. (1987) The distribution of four lysosomal integral membrane proteins (LIMPs) in rat basophilic leukemia cells.Tissue Cell. 19: 495-504.4. La Rosa, S.et al.(2011) Histologic characterization and improved prognostic evaluation of 209 gastric neuroendocrine neoplasms.Hum Pathol. 42: 1373-84.5. Takeda, A.et al.(2015) Axonal regeneration through the fibrous scar in lesioned goldfish spinal cord.Neuroscience. 284: 134-52.6. Cuello, A.C.et al.(1982) Immunocytochemistry with internally labeled monoclonal antibodies.Proc Natl Acad Sci U S A. 79: 665-9. |
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