Sheep anti methotrexate antibody
货号：6120-0004
规格：0.5 ml
价格：2576
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：methotrexate
物种：Chemical
宿主：Sheep
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Sheep anti methotrexate antibodyrecognizes Methotrexate, a drug originally manufactured as a chemotherapeutic agent for the treatment or a number of cancers. Methotrexate also acts as a disease-modifying anti-rheumatic drug used for the treatment of autoimmune conditions such as rheumatoid arthritis, Chron's disease and vasculitis.Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-Aminopterin 18%Folic acid <0.01%Dyhydrofolic acid <0.01%Tetrahydrofolic acid<0.01%N-5-Methyltetrahydrofolic acid<0.01%Folinic acid<0.01%N-(p-Aminobenzoyl)-L-glutamic acid<0.01% |
| 产品详情 | | |
Target Species | Chemical |
Product Form | Serum - liquid |
Antiserum Preparation | <0.1% Sodium Azide (NaN3) |
Immunogen | Methotrexate (4-amino-10-methylpteroyl-L-glutamicMethotrexate (4-amino-10-methylpteroyl-L-glutamic acid) conjugated via the carboxyl groups of the glutamic acid residue to KLH. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;P;WB | 1.5 ml |
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F;IF | 0.1 mg |
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
| 文献 |
| 1. Suzuki, Y.et al.(2003) The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection.Anal Sci. 19 (1): 111-5.2. Tesfalidet, S.et al.(2016) Detection of methotrexate in a flow system using electrochemical impedance spectroscopy and multivariate data analysis.Anal Chim Acta. 914: 1-6.3. Sereilakena, Pet al.(2017) Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysisAnalytica Chimica Acta. 987: 15-24.4. Phal, S.et al.(2018) Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexateJournal of Electroanalytical Chemistry. Jan 3 [Epub ahead of print].5. Mwanza, D.et al.(2021) Electrografting of isophthalic acid monolayer and covalent attachment of antibody onto carbon surfaces: construction of capacitive biosensor for methotrexate detectionElectrochimica Acta. : 139360. [Epub ahead of print] |
技术参数 产品应用 ELISA
产品类型 Polyclonal Antibody
推荐用于 ELISA
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