| 产品介绍 | | |
| Mouse anti Human CD68 antibody, clone 514H12recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes. |
| 产品详情 | | |
Target Species | Human |
| Product Form | TissueCulture Supernatant - liquid |
Preservative Stabilisers | Pack Size: 1 ml 0.09% Sodium Azide Pack Size: 0.1 ml <0.1% Sodium Azide (NaN3) |
| Immunogen | Fusion protein corresponding to external domain of human CD68. |
| Fusion Partners | Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin1 | √ | 1/40 | 1/80 |
| Immunofluorescence | √ | | |
| Western Blotting | x | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.N.B. The epitope recognised by clone 514H12 is sensitive to peroxide. It is therefore recommended that any peroxidase-blocking steps be performed after incubation with this antibody.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control TissueHuman tonsil
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| 数据 |
| Detection of Mouse anti Human CD68 (MCA1815) in an immunohistochemical analysis of formalin fixed, paraffin embedded tonsil section following heat mediated antigen retrieval with TRIS-EDTA buffer, pH9.0. |
| 文献 |
| 1. Quispel, W.T.et al.(2016) Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis.Oncoimmunology. 5 (8): e1164364.2. Toh, M.L.et al.(2014) Bone- and cartilage-protective effects of a monoclonal antibody against colony-stimulating factor 1 receptor in experimental arthritis.Arthritis Rheumatol. 66 (11): 2989-3000.3. Schirmer, L.et al.(2014) Differential loss of KIR4.1 immunoreactivity in multiple sclerosis lesions.Ann Neurol. 75 (6): 810-28.4. Schrevel, M.et al.(2017) Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer.Int J Oncol. 50 (6): 1947-54.5. Zhang, W.et al.(2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.PLoS One. 8 (5): e65121.6. Bobosha, K.et al.(2014) T-cell regulation in lepromatous leprosy.PLoS Negl Trop Dis. 8 (4): e2773.7. van Poppel, P.C.M.et al.(2018) Inflammation in the subcutaneous adipose tissue does not attenuate endothelial function in subjects with diabetes mellitus and subjects with dyslipidaemia and hypertension: Across-sectional study.Endocrinol Diabetes Metab. 1 (3): e00020.8. Yuan, A.et al.(2020) Histologic analysis of medication-related osteonecrosis of the jaw compared with antiresorptive-exposed bone and other infectious, inflammatory, and necrotic jaw diseases.Oral Surg Oral Med Oral Pathol Oral Radiol. 129 (2): 133-40.9. Durán, D.et al.(2020) Histological and Immunohistochemical Study of Wounds in Sheep Skin in Maggot Therapy by UsingProtophormia terraenovae.(Diptera:Calliphoridae.) Larvae.J Med Entomol. 57 (2): 369-76. |
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