产品介绍 | | |
Rabbit antiListeria monocytogenesantibodyreacts withListeria monocytogeneswhole cells. Rabbit antiListeria monocytogenesantibody has not been absorbed and may react with related micro-organisms.Rabbit antiListeria monocytogenesantibody has been used successfully for the demonstration of internalizedL. monocytogenesin formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salemet al.2015). |
产品详情 | | |
Target Species | Bacterial |
Product Form | Purified IgG - liquid |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | 4.0 mg/ml |
Immunogen | Listeria monocytogenes.whole cells. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunohistology - Paraffin1 | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
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Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
数据 |
Published customer image:Rabbit antiListeria monocytogenesantibody (5688-1990) used to stainListeria monocytogenesin CD4+T cells by immunofluorescence.Image caption:Orthogonal views of Listeria tpCD4+T cells purified at 3 h of conjugate formation. Actin is shown in magenta, andListeriain green. XY together with YZ and XZ views (from lines marked in XY) are shown. Bar = 10μm.From: Cruz-Adalia A, Ramirez-Santiago G, Osuna-Pérez J, Torres-Torresano M, Zorita V, Martínez-Riaño A, Boccasavia V, Borroto A, Martínez Del Hoyo G, Gozález-Granado JM, Alarcón B, Sánchez-Madrid F, Veiga E.Conventional CD4(+)T cells present bacterial antigens to induce cytotoxic and memory CD8(+)T cell responses.Nat Commun. 2017 Nov 17;8(1):1591. |
文献 |
1. Marusov, G.et al.(2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.Environ Sci Technol. 46 (1): 348-59.2. Salem Met al.(2015) Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn's associated NOD2 gene variants.Clin Exp Immunol. 179 (3): 426-34.3. Témoin, S.et al.(2008) Multiple point mutations in virulence genes explain the low virulence ofListeria monocytogenesfield strains.Microbiology. 154 (Pt 3): 939-48.4. Cruz-Adalia, A.et al.(2017) Conventional CD4+T cells present bacterial antigens to induce cytotoxic and memory CD8+T cell responses.Nat Commun. 8 (1): 1591.5. Okita, Y.et al.(2016) Interleukin-22-Induced Antimicrobial Phospholipase A2 Group IIA Mediates Protective Innate Immunity of Nonhematopoietic Cells againstListeria monocytogenes.Infect Immun. 84 (2): 573-9. |