Mouse anti Pig CD203a, clone PM18-7
货号：MCA1973F
规格：0.1 mg
价格：3740
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD203a
物种：pig
宿主：小鼠
抗体亚型：IgG1
克隆号：PM18-7
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Pig CD203a, clone PM18-7recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguezet al.1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 orENPP1(Petersenet al.2007).Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguezet al.1998) under both reducing and non-reducing conditions. CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersenet al.2007) from preparations of porcine alveolar macrophages.CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersenet al.2007,Hwanget al.2015), it is not expressed on monocyte populations (McCulloughet al.1997,Hwanget al.2015).SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchezet al.1999). |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | Porcine alveolar macrophages. |
| Fusion Partners | Spleen cells from immunized mice were fused with P3X63-Ag8-653 murine myeloma cells (Kearneyet al.1979). |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.McCullough, K.C.et al.(1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.Vet Immunol Immunopathol. 58 (3-4): 265-75.2.McCullough, K.C.et al.(1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.Immunology. 98 (2): 203-12.3.Boersma, W.J.et al.(2001) Summary of workshop findings for porcine B-cell markers.Vet Immunol Immunopathol. 80 (1-2): 63-78. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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