Native Escherichia coli Beta Galactosidase
货号：4600-1704
规格：5 mg
价格：3460
产品类型：蛋白
品牌：Bio-Rad
| 产品介绍 | | |
| Escherichia coliBeta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. |
| 产品详情 | | |
Target Species | Bacterial |
Product Form | Purified protein fromEscherichia coli- lyophilized |
Reconstitution | Reconstitute with 1.0 ml sterile distilled water or aqueous buffer of choice.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | Contains Sucrose |
Approx. Protein Concentrations | Protein content is approximately 20-25% of total weight |
Activity | >600 U/mg proteinOne unit hydrolyses 1 µmole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.For batch-specific activity, please inquire. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Protein Molecular Weight | 540 kDa |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Functional Assays | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
技术参数 产品应用 FN
产品类型 Purified Protein
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