Mouse anti Rat CD25 antibody, clone OX-39
货号：MCA273PE
规格：100 Tests
价格：3740
产品类型：流式抗体
品牌：Bio-Rad
物种：大鼠
宿主：小鼠
抗体亚型：IgG1
克隆号：OX-39
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Rat CD25 antibody, clone OX-39recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Patersonet al.1987). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid |
| Reconstitution | Reconstitute with 1.0ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS0/1 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
| 文献 |
| 1.Paterson, D.J.et al.(1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.Mol Immunol. 24 (12): 1281-90.2.Charteris DG & Lightman SL (1993)In vivolymphokine production in experimental autoimmune uveoretinitis.Immunology. 78 (3): 387-92.3.Hayosh, N.S. & Swanborg, R.H. (1987) Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors.J Immunol. 138 (11): 3771-5. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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