| 产品介绍 | | |
| Native human Ferritinis a purified preparation of human ferritin from liver tissue. Ferritin is a globular protein composed of 24 identical subunits responsible for storing iron in an available non-toxic form. Ferritin is found primarily in hepatic tissue. Serum levels of ferritin are used as an indicator for anaemia and restless leg syndrome. Ferrin levels are a direct correlate for the total amount of irron stored in the body |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified protein from human liver - liquid |
Preparation | Multiple purification steps |
Buffer Solution | TRIS buffered sodium chloride pH 7-8 |
Preservative Stabilisers | <0.01% bromo-nitro-dioxane<0.01% methylisothiazolone |
Approx. Protein Concentrations | 3.0 mg/ml |
Purity | SDS PAGE: >95% |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 数据 |
| Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F23 (4420-3010) as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7(4420-3904) as a detection reagent with purified human ferritin (4420-4804) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted |
| 文献 |
| 1. Addison, J.M.et al.(1983) The amino acid sequence of human liver apoferritin.FEBS Lett. 164 (1): 139-44.2. Na, Y.et al.(2019) Carbon nanotube facilitated interface formation for enhanced protein sensing in electrosynthesized molecular imprintingACS Applied Bio Materials. Aug 29 [Epub ahead of print]. |
QQ:1749072012