Rat anti mouse IgG3 HEAVY chain
货号：MCA423
规格：0.25 mg
价格：3018
产品类型：二抗
品牌：Bio-Rad
抗原：IgG3
物种：小鼠
宿主：大鼠
抗体亚型：IgM
克隆号：LO-MG3-7
荧光染料：Purified
| 产品介绍 | | |
| Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7recognizes the gamma 3 heavy chain of mouse IgG and does not cross react with other subclasses of murine IgG. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgM - liquid |
| Preparation | Purified IgM prepared by affinity chromatography from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.1%Sodium Azide |
| Immunogen | Polyclonal and monoclonal mouse IgG3. |
| Approx. Protein Concentrations | IgM concentration 1 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.Prot Biol Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-8.2.Bazin, H.et al.(1984) Rat monoclonal antibodies. I. Rapid purification fromin vitroculture supernatants.J Immunol Methods. 66 (2): 261-9.3.Bazin, H.et al.(1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.J Immunol Methods. 71 (1): 9-16.4.Zivkovic, I.et al.(2012) Induction of decreased fecundity by tetanus toxoid hyper-immunization in C57BL/6 mice depends on the applied adjuvant.Innate Immun. 18: 333-42. |
技术参数 产品应用 ELISA
产品类型 Monoclonal Antibody
推荐用于 ELISA
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